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ELYSIA GRANDIFOLIA
Cell Culture
Culture vessels, plates, dishes, strainers, and maintenance tools for mammalian and microbial cell culture workflows.
T25–T225
Flask Sizes
TC Treated
Surface
Sterile
Individually Wrapped
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Choosing the Right Culture Vessel
Culture flasks are the workhorse for expanding adherent and suspension cells — choose by growth area (T25 for small-scale, T225 for large-scale production). Multi-well plates are used for assays, drug screening, and experiments requiring multiple conditions in parallel. Petri dishes work for microbiology, colony picking, and short-term cell observation. All our culture vessels are TC-treated, sterile, and individually wrapped.
FLASK SIZE GUIDE
Which flask size do you need?
| Flask | Growth Area | Media Volume | Best For |
|---|---|---|---|
| T25 | 25 cm² | 5–7 mL | Thawing cells, small-scale maintenance, primary cultures |
| T75 | 75 cm² | 15–20 mL | Routine cell maintenance, most common flask size |
| T150 | 150 cm² | 30–50 mL | Scale-up before experiments, medium production runs |
| T225 | 225 cm² | 45–70 mL | Large-scale production, high cell yield requirements |
💡Vented vs. Plug Seal Caps
Vented caps have a 0.22µm membrane that allows gas exchange (CO₂/O₂) while maintaining sterility — use these in CO₂ incubators. Plug seal caps create a tight seal for transport or when growing cells without CO₂ (some bacterial cultures). Most mammalian cell culture uses vented caps.
COMMON QUESTIONS
Cell Culture FAQ
Yes. All flasks ship individually wrapped, gamma-irradiated, and certified sterile. Lot-specific sterility certificates are available on request.
Use 40µm for preparing single-cell suspensions for flow cytometry. 70µm is the most versatile for general tissue dissociation. 100µm is best for loosely dissociated tissues where you want to minimize cell damage.
Our standard flasks are TC-treated for adherent cells. For suspension cultures (CHO, Jurkat, etc.), you can use our flasks without TC treatment or contact us for ultra-low attachment options.
TC (tissue culture) treated surfaces are modified to be hydrophilic, which promotes cell attachment and spreading. Untreated surfaces are hydrophobic and work better for suspension cultures or non-adherent cell types.
Setting up a cell culture facility?
We carry flasks, plates, media, and specialty beads for your cell biology workflows.