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GLAUCUS ATLANTICUS
Extraction & Purification
Column-based kits, magnetic bead systems, and complete workflows for DNA, RNA, plasmid, and protein extraction. Isolate your target analyte with high purity and reproducible yields.
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Extraction Platforms
High Yield
Optimized Protocols
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What are you extracting?
Choose your extraction platform based on your target analyte, sample type, and throughput requirements.
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Choosing an Extraction Method
Nucleic acid and protein extraction is the critical first step in any molecular workflow. Silica column kits use spin columns with chaotropic lysis buffers to bind DNA or RNA to a silica membrane, wash away contaminants, and elute pure nucleic acid in minutes. Magnetic bead kits use paramagnetic particles coated with nucleic acid-binding surfaces, making them ideal for automation and high-throughput processing on robotic platforms. Organic extraction (phenol-chloroform) remains the gold standard for maximum yield from difficult samples but requires more hands-on time and chemical handling.
EXTRACTION METHODS
Column vs. bead vs. organic extraction
| Method | Mechanism | Throughput | Yield | Best For |
|---|---|---|---|---|
| Silica Column | Chaotropic lysis + silica membrane binding | 1-24 samples/run | Moderate-High | Routine bench-scale extractions, clinical samples |
| Magnetic Bead | Paramagnetic particle capture | 24-96 samples/run | Moderate | Automation, HT screening, plate-based workflows |
| Organic (Phenol-Chloroform) | Liquid-liquid phase separation | 1-12 samples/run | Highest | Difficult samples, high-MW gDNA, maximum yield |
💡Quick Selection Guide
Routine DNA/RNA from blood, tissue, or cells? Start with a silica column kit - fast, reproducible, and no organic solvents. Processing 48+ samples at once? Magnetic bead kits in 96-well format are the most efficient choice, especially if you have a liquid handler. Need maximum yield from tough samples (plant tissue, soil, FFPE)? Consider organic extraction or specialized kits designed for those matrices. For plasmid prep, match your kit scale (mini, midi, maxi) to the volume of culture you are processing.
COMMON QUESTIONS
Extraction & Purification FAQ
These refer to the scale of plasmid DNA purification. A miniprep processes 1-5 mL of bacterial culture and yields 5-20 ug of plasmid DNA - suitable for sequencing, cloning, and restriction analysis. A midiprep handles 25-100 mL of culture (100-200 ug yield) for transfection-grade DNA. A maxiprep processes 100-500 mL of culture (500-2000 ug yield) for large-scale transfections and in vivo work.
Standard column kits are optimized for fresh or frozen tissue. For formalin-fixed, paraffin-embedded (FFPE) samples, you need kits with extended proteinase K digestion and de-crosslinking steps. Contact us for specific FFPE-compatible kit recommendations.
Yes. Our magnetic bead extraction kits are designed for 96-well plate format and are compatible with common robotic platforms including KingFisher, Hamilton STAR, Tecan, and Beckman Biomek. The protocols can be adapted to your specific instrument.
Pure DNA should give an A260/A280 ratio of approximately 1.8. Pure RNA should be approximately 2.0. Ratios significantly below these values indicate protein contamination. Ratios above 2.0 for DNA may indicate RNA contamination. Our kits routinely achieve these purity benchmarks when protocols are followed correctly.
Not sure which extraction kit fits your workflow?
Our molecular biology team can help you select the right kit based on your sample type, throughput, and downstream application.