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PHYLLIDIA VARICOSA
Buffers & Solvents
Ready-to-use biological buffers, pH calibration standards, analytical-grade solvents, and purified water for chromatography, molecular biology, and general lab use.
ACS Grade
Solvent Purity
NIST-Traceable
pH Standards
Lot CoA
Available
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Selecting the Right Grade for Your Application
HPLC / LC-MS grade solvents have the lowest UV absorbance and lowest extractables — essential for chromatographic separations where background interference affects detection limits. ACS grade meets American Chemical Society purity standards and works for general analytical chemistry. Molecular biology grade reagents are tested for nuclease contamination and are suitable for PCR, cloning, and sequencing workflows. Always match the reagent grade to your application's sensitivity requirements.
GRADE COMPARISON
Which grade do you need?
| Grade | Purity | Best For | Key Specs |
|---|---|---|---|
| HPLC Grade | ≥99.9% | HPLC, GC, LC-MS mobile phases | Low UV cutoff, low residue |
| ACS Grade | ≥99.5% | General chemistry, titrations, extractions | Meets ACS reagent specs |
| Molecular Biology | Varies | PCR, gel electrophoresis, cloning | DNase/RNase/protease free |
| NIST-Traceable | Certified | pH meter calibration, QC checks | Certificate of analysis included |
💡Storage Matters
Store solvents in a cool, dry area away from direct sunlight. Once opened, HPLC-grade solvents should be used within 6 months for best chromatographic performance. pH standards should be used within 12 months of opening and kept tightly sealed to prevent CO₂ absorption.
COMMON QUESTIONS
Buffers & Solvents FAQ
HPLC-grade methanol has higher purity (≥99.9%), lower UV absorbance, and lower non-volatile residue. It's specifically tested for chromatographic performance. ACS-grade (≥99.5%) meets general analytical standards but may have trace impurities that affect sensitive detectors like mass spectrometry.
Yes. All NIST-traceable pH calibration standards ship with a lot-specific certificate of analysis that includes measured values and uncertainty data.
Yes, but solvents ship ground only due to flammability. We carry 500mL, 1L, 2.5L, and 4L sizes. Contact us for pallet-quantity pricing on high-volume solvents.
Yes. Our molecular biology water is tested and certified DNase-free, RNase-free, and protease-free. It's 0.1µm filtered and meets the purity standards for PCR, RT-qPCR, and sequencing.
Running through chemicals fast?
Ask about volume pricing on cases of solvents and bulk buffer orders.