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ELYSIA GRANDIFOLIA
Cell Culture Flasks
TC-treated culture flasks from T-25 to T-225. Vented and plug seal caps with canted-neck design for optimal gas exchange and media handling.
25–225 cm²
Growth Areas
2
Cap Types
TC-Treated
Surface
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TC-Treated Flasks for Adherent Cell Culture
Our cell culture flasks feature vacuum-gas plasma TC treatment that creates a hydrophilic, negatively charged surface for optimal cell attachment. Available in T-25, T-75, T-150, and T-225 formats with canted necks that allow complete media removal and reduce contamination risk during handling.
FLASK COMPARISON
T-25 vs T-75 vs T-150 vs T-225
| Flask | Growth Area | Working Volume | Typical Cell Yield | Best For |
|---|---|---|---|---|
| T-25 | 25 cm² | 5–10 mL | 2–5 × 10⁶ | Small-scale culture, primary cells, assay setup |
| T-75 | 75 cm² | 15–20 mL | 8–15 × 10⁶ | Routine passaging, cell line maintenance |
| T-150 | 150 cm² | 30–50 mL | 20–40 × 10⁶ | Scale-up, protein production, virus amplification |
| T-225 | 225 cm² | 45–70 mL | 40–70 × 10⁶ | Large-scale harvest, high-demand workflows |
💨Vented vs. Plug Seal Caps
Vented caps have a 0.22 µm hydrophobic membrane that allows CO₂/O₂ gas exchange while maintaining sterility—use these in standard CO₂ incubators. Plug seal caps create an airtight seal for transport, closed-system culture, or when using sealed-flask protocols without CO₂ supplementation.
🔬What Does TC-Treated Mean?
TC (tissue culture) treatment modifies the polystyrene surface using vacuum-gas plasma to increase hydrophilicity and introduce charged functional groups. This promotes cell attachment, spreading, and proliferation for adherent cell lines. Non-treated flasks are available for suspension culture applications.
FREQUENTLY ASKED
Culture Flask FAQ
Use vented caps for standard incubator culture where CO₂ exchange is needed. Use plug seal caps for transport, storage, or closed-system protocols. Most routine cell culture uses vented caps.
TC-treated flasks maintain surface properties for 3–5 years when stored in original packaging at room temperature. Check the lot-specific expiration date printed on each case.
TC-treated surfaces promote cell attachment, which is not ideal for suspension culture. For suspension cells, use non-treated flasks or ultra-low attachment surfaces to prevent unwanted adhesion.
Typical seeding densities range from 5,000–20,000 cells/cm² depending on doubling time. For a T-75, that means roughly 0.4–1.5 × 10⁶ cells. Consult your cell line’s ATCC datasheet for specific recommendations.
Scaling up your cell culture operation?
We offer case pricing on culture flasks with consistent lot-to-lot TC treatment. Request a quote for your monthly usage.