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HYPSELODORIS TRICOLOR
PCR Master Mixes
Ready-to-use PCR and qPCR master mixes with Taq or high-fidelity polymerases. Hot-start and standard formulations for endpoint, real-time, and cloning applications.
2×
Concentration
Hot-Start
Available
qPCR
& Endpoint
2x RT-qPCR Master Mix, 1mL
SKU: PCR-MM-0001
🧬
Pre-Formulated Mixes for Consistent PCR
Our 2× master mixes contain polymerase, dNTPs, MgCl₂, and optimized buffer in a single tube. Just add primers, template, and water. Pre-formulated mixes reduce pipetting steps, minimize variability between reactions, and speed up setup time—especially valuable for multi-plate experiments.
💡Hot-Start vs. Standard Polymerase
Hot-start polymerases are chemically modified or antibody-inhibited to remain inactive below 90°C. This prevents primer-dimer and non-specific amplification during room-temperature setup. Hot-start is recommended for multiplex PCR, low-copy targets, and any application where specificity matters. Standard (non-hot-start) mixes are more economical for simple, high-copy amplifications.
🛡️When to Use High-Fidelity Mixes
High-fidelity polymerases have proofreading (3’→5’ exonuclease) activity that reduces error rates 50–100× compared to standard Taq. Use high-fidelity mixes for cloning, site-directed mutagenesis, sequencing library prep, and any application where sequence accuracy is critical. Note: high-fidelity polymerases generate blunt-ended products, not A-tailed.
FREQUENTLY ASKED
Master Mix FAQ
Mix equal volumes of 2× master mix and your primer/template/water combination. For a 20 µL reaction: 10 µL master mix + 10 µL of your primer/template/water mix. The final reaction contains 1× buffer, polymerase, dNTPs, and MgCl₂ at optimized concentrations.
No. Our master mixes include MgCl₂ at an optimized concentration (typically 1.5–2.0 mM final). Additional MgCl₂ is only needed for specific optimization of difficult targets and is not recommended as a default.
Use our SYBR Green or probe-based qPCR master mixes for real-time quantification. Standard Taq master mixes are formulated for endpoint PCR and do not contain fluorescent dyes or passive reference dyes (ROX) needed for qPCR instruments.
Store at −20°C for long-term storage. Most mixes are stable at 4°C for 1–2 weeks for active use. Avoid repeated freeze-thaw cycles—aliquot into single-use volumes if you run PCR infrequently.
No. High-fidelity polymerases produce blunt-ended products that are incompatible with TA cloning vectors. For TA cloning, use standard Taq-based master mix which adds a 3’ A-overhang. Alternatively, A-tail your blunt-ended product with Taq before ligation.
Need help choosing the right mix?
Our technical team can recommend the optimal master mix for your target, instrument, and throughput requirements.