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GLAUCUS ATLANTICUS
DNA/RNA Buffers
Lysis, binding, wash, and elution buffers for column-based and magnetic bead DNA/RNA extraction workflows. Compatible with Filtrous extraction kits and standard silica membrane protocols.
12
Buffer Formulations
0.5–1L
Bottle Sizes
Matched
Kit Compatible
Universal Column DNA/RNA Extraction Kit, 50 Preps/unit
SKU: EXK-CO-0050
Universal Magnetic DNA/RNA Extraction Kit, 96 Preps/unit
SKU: EXK-BD-0096
Buffer CLY, 100 Preps/unit
SKU: EXK-CL-0100
Buffer N3, 0.5L
SKU: QBE-N3-0500
Buffer NLY, 100 Preps/unit
SKU: EXK-NL-0100
Buffer P1, 0.5L
SKU: QBE-PX-0500
Buffer P2, 0.5L
SKU: QBE-PY-0500
Buffer P3, 0.5L
SKU: QBE-PZ-0500
Buffer PB, 0.5L
SKU: QBE-PB-0500
Buffer QBT, 1L
SKU: QBE-QB-1000
Buffer QC, 1L
SKU: QBE-QC-1000
Buffer QF, 1L
SKU: QBE-QF-1000
Buffer QN, 0.5L
SKU: QBE-QN-0500
🧬
Extraction Buffers for Column and Bead Workflows
Each step of nucleic acid purification requires a specific buffer chemistry. Lysis buffers (P1, P2, N3) break open cells and denature proteins. Binding buffers (PB) create the chaotropic conditions that drive DNA/RNA onto silica membranes or magnetic beads. Wash buffers (QC) remove salts, proteins, and other contaminants. Elution buffers (QF) release purified nucleic acid from the column. These buffers are sold individually for labs that need to replenish specific reagents without reordering a full kit.
🛡️Buffer Storage
Store buffers at room temperature (15–25°C) unless the label specifies otherwise. Buffer P1 (resuspension buffer) should be stored at 2–8°C after adding RNase A. Check for precipitates before use — warm to 37°C briefly to redissolve if needed. Buffers containing SDS (P2, lysis buffers) may precipitate below 15°C.
FREQUENTLY ASKED
DNA/RNA Buffer FAQ
Yes. All of our extraction buffers are available as standalone bottles. This is useful when you run out of a specific buffer before other kit components, or when you need extra wash or elution buffer for modified protocols with additional wash steps.
Our buffers are formulated for use with Filtrous extraction kits and columns, but the underlying chemistry (guanidinium-based lysis, isopropanol binding, ethanol wash) is standard across silica membrane platforms. Many customers use them with compatible third-party columns.
Most buffers have a 2-year shelf life from date of manufacture when stored at room temperature in sealed containers. Buffer P1 with RNase A added should be stored at 2–8°C and used within 6 months of reconstitution.
P-series buffers (P1, P2, P3, PB) are used in miniprep and standard column-based extraction workflows. Q-series buffers (QBT, QC, QF, QN) are used in anion-exchange purification protocols (midiprep and maxiprep scale) where larger volumes and gravity-flow columns are used.
Need buffers in bulk or custom formulations?
We offer volume pricing on individual buffers and can help you identify the right reagents for your extraction workflow.