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Flushing an HPLC column is crucial for its durability and optimal performance. This blog post presents two techniques to extend the column's life: daily maintenance using solvents or manual cleaning, and a more thorough reverse flush. Monitoring pressure buildup helps determine when a reverse flush is necessary. By employing these techniques, users can save costs and avoid premature column replacement.

Calibration curves are essential in analytical chemistry to determine the concentration of unknown samples. When using instruments like an HPLC, the absorbance reading alone does not provide concentration information. To construct a calibration curve, known standards are injected into the instrument, and their results are compared to unknown samples. By extrapolating the values, the concentration of the unknown samples can be determined. The process involves creating stock solutions, diluting them to desired concentrations, injecting them into the instrument, and constructing the calibration curve. Calibration curves provide valuable information about the samples being analyzed.

This blog discusses the process of testing the potency of cannabis flowers using High-Performance Liquid Chromatography (HPLC). Testing the potency of cannabis flower is important to determine the concentration of cannabinoids like THC and CBD, ensuring a consistent and reliable product. The steps involved in testing potency include homogenizing the sample, weighing out 200 milligrams of the sample, diluting the sample with methanol, further diluting the sample, filtering the sample to remove solid particles, and preparing the sample for HPLC analysis. By following these steps, you can accurately determine the potency of your cannabis flower.

This blog provides a brief summary of the steps involved in preparing a well plate for qPCR experiments. It emphasizes the importance of keeping ingredients on ice for optimal temperature. The key steps are plating the master mix, adding samples with fresh pipette tips, sealing the plate with optical seals, spinning it down to secure the seal and remove air bubbles, and storing it on ice. Tips for success include using filter tips, following vendor instructions, and using high-quality tools. The blog highlights their company's range of products for well plate experiments.

This blog post provides a comprehensive guide to calibrating and using a balance in laboratory work. It emphasizes the importance of precision and accuracy in measurements and outlines the steps involved in setting up and using a balance. The post covers topics such as calibrating the balance using pre-calibrated weight standards, leveling the balance, performing a calibration, and understanding the "zero" and "tare" functions. By following these guidelines, scientists, researchers, and students can ensure reliable and precise measurements in their work.

This blog post provides researchers and cell culturists with essential knowledge on determining when cells are ready to be subcultured. It explores various methods for assessing cell confluency, including visual inspection, measuring the percentage of covered area, counting cells, and using staining dyes. The importance of considering cell type, culture conditions, and experience in determining confluency is highlighted. The step-by-step guide and checklist provided offer a practical approach to ensure accurate assessment and successful subculturing of cells, ultimately maintaining healthy and productive cell cultures.

This blog post provides an introduction to High Pressure Liquid Chromatography (HPLC). HPLC is a technique used for separating and identifying components in substances. The post explains that HPLC involves a stationary phase (such as a column) and a mobile phase (the liquid solution). It discusses the separation mechanism, focusing on reverse-phase HPLC, which uses a polar mobile phase and a nonpolar column. The post compares the separation process to a water slide, where polar molecules come out faster due to less attraction, while nonpolar molecules come out slower due to more attraction.

This blog provides a brief summary of selecting the right HPLC vials for laboratory testing needs. It emphasizes the importance of considering factors such as autosampler compatibility, sample volume, sample compatibility, vial composition, glass type, autosampler vial material, caps, septa, and proper inserts. The blog also highlights the significance of choosing the appropriate color, closure, and storage method for the vials to ensure accurate results and prevent sample degradation. Lastly, it mentions the drawbacks of re-using vials and emphasizes the need for avoiding contamination.