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20X SSPE Buffer, pH 7.4, 1L

SKU: SSP-20-0100
$38.85

A hybridization and wash buffer commonly used in Southern blotting, Northern blotting, and nucleic acid hybridization assays. This formulation provides optimal ionic strength for stable nucleic acid duplex formation and efficient probe binding. The buffer allows precise stringency control through dilution, enabling detection of both highly homologous and partially matched sequences. Compatible with radioactive, fluorescent, and chemiluminescent detection systems. Prepared with high-purity reagents for reliable hybridization results.

Specifications

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Specification Value
Concentration 20X
Name SSPE Buffer
pH at 1x pH 7.4
Volume 1L
SKU SSP-20-0100
Composition at 1x NaCl 3.0 M, NaH2PO4 0.2 M, EDTA 0.02 M

A hybridization and wash buffer commonly used in Southern blotting, Northern blotting, and nucleic acid hybridization assays. This formulation provides optimal ionic strength for stable nucleic acid duplex formation and efficient probe binding. The buffer allows precise stringency control through dilution, enabling detection of both highly homologous and partially matched sequences. Compatible with radioactive, fluorescent, and chemiluminescent detection systems. Prepared with high-purity reagents for reliable hybridization results.

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