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20X SSPE Buffer, pH 7.4, 1L
A hybridization and wash buffer commonly used in Southern blotting, Northern blotting, and nucleic acid hybridization assays. This formulation provides optimal ionic strength for stable nucleic acid duplex formation and efficient probe binding. The buffer allows precise stringency control through dilution, enabling detection of both highly homologous and partially matched sequences. Compatible with radioactive, fluorescent, and chemiluminescent detection systems. Prepared with high-purity reagents for reliable hybridization results.
Specifications
| Specification | Value |
|---|---|
| Concentration | 20X |
| Name | SSPE Buffer |
| pH at 1x | pH 7.4 |
| Volume | 1L |
| SKU | SSP-20-0100 |
| Composition at 1x | NaCl 3.0 M, NaH2PO4 0.2 M, EDTA 0.02 M |
A hybridization and wash buffer commonly used in Southern blotting, Northern blotting, and nucleic acid hybridization assays. This formulation provides optimal ionic strength for stable nucleic acid duplex formation and efficient probe binding. The buffer allows precise stringency control through dilution, enabling detection of both highly homologous and partially matched sequences. Compatible with radioactive, fluorescent, and chemiluminescent detection systems. Prepared with high-purity reagents for reliable hybridization results.
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