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Specification |
Value |
Capacity |
50ug of plasmid DNA |
Volume |
Purify from 1 to 4mL of E. coli culture, yielding 8-12ug from 1mL culture |
Content |
Volume |
Preps | 50 preps/unit |
Mini Spin Column I | 250 |
Buffer AA | 65mL |
Buffer BA | 65mL |
Buffer NA | 90mL |
Buffer KB | 70mL |
DNA Wash Buffer | 50mL |
Elution Buffer | 30mL |
RNase A | 210uL |
Isolation and Purification of plasmid DNA
Plasmid extraction kits are essential tools in molecular biology laboratories, facilitating the isolation and purification of plasmid DNA from bacterial cultures.
Methods
These kits typically employ spin column-based or silica membrane-based methods to efficiently separate plasmid DNA from genomic DNA, RNA, and other cellular components.
Applications
The purified plasmid DNA can then be used for a variety of downstream applications, including cloning, PCR amplification, sequencing, and transfection into other cells.
Easy Process with High-quality Results
Plasmid extraction kits streamline the process, yielding high-quality DNA with minimal contamination, enabling researchers to reliably study gene expression, protein production, and genetic engineering experiments.