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Specification |
|
Specification | Value |
Capacity | 250ug of plasmid DNA |
Quantity | Purify from 15 to 50mL of E. coli culture, yielding 150-250ug from 50mL culture |
Kit Content |
|
Kit Content | Quantity |
Midi Spin Column | 25 |
Buffer AA | 70mL |
Buffer BA | 70mL |
Buffer CA | 90mL |
DNA Wash Buffer | 50mL |
RNase A | 7mg |
Elution Buffer | 30mL |
Isolate and Purify Plasmid DNA
Plasmid extraction kits are essential tools in molecular biology laboratories, facilitating the isolation and purification of plasmid DNA from bacterial cultures. These kits typically employ spin column-based or silica membrane-based methods to efficiently separate plasmid DNA from genomic DNA, RNA, and other cellular components.
Application
The purified plasmid DNA can then be used for a variety of downstream applications, including cloning, PCR amplification, sequencing, and transfection into other cells.
Reliable DNA Extraction
Plasmid extraction kits streamline the process, yielding high-quality DNA with minimal contamination, enabling researchers to reliably study gene expression, protein production, and genetic engineering experiments. They are indispensable in both academic research and industrial biotechnology settings.