Universal Wound qPCR detection Kit, 100 Rxn/ Kit

Name: Universal Wound qPCR detection Kit, 100 Rxn/ Kit
Brand: Filtrous
SKU: OSK-WD-0100
Price: 4800.0 USD
Availability: InStock

SKU OSK-WD-0100

by Filtrous

$4,800.00 - $4,800.00

$4,800.00

$4,800.00 - $4,800.00

$4,800.00

Specifications

Specification	Value
Reactions	100 reactions per unit
Targets	Morganella morganii Klebsiella pneumoniae Staphylococcus aureus Enterobacter cloacae Enterococcus faecalis Proteus mirabilis Klebsiella oxytoca Pseudomonas aeruginosa Escherichia coli Serratia marcescens Providencia stuartii Acinetobacter baumannii Bacteroides spp. Citrobacter freundii Citrobacter braakii Klebsiella aerogenes Enterococcus faecium Proteus vulgaris Streptococcus pyogenes
Shelf Life	12 months from the date of manufacture; store frozen at -20°C ± 5°C
Intended use	Available for research use only (RUO). Not for use in diagnostic procedures.

Features and Benefits

Ultra-High Sensitivity: Capable of detecting target concentrations as low as a few copies per reaction, enabling the detection of rare mutations or low-abundance transcripts with unparalleled sensitivity.
Exceptional Specificity: Incorporates proprietary primer and probe design algorithms and stringent reaction conditions to ensure minimal cross-reactivity and off-target amplification, resulting in highly specific and reliable results.
Rapid Turnaround Time: Optimized reaction protocols and thermal cycling conditions enable rapid amplification and detection, delivering results in significantly reduced assay times compared to conventional qPCR assays.
Multiplexing Capability: Designed for seamless multiplexing of multiple targets within a single reaction, maximizing throughput and efficiency without compromising sensitivity or specificity.
Integrated Quality Controls: Features built-in internal controls and reference genes to monitor assay performance, identify potential inhibitors or sample degradation, and ensure consistent and reliable results.
High Compatibility: Compatible with a wide range of qPCR instruments, ensuring easy integration into existing workflows and protocols without the need for specialized equipment or reagents.
Comprehensive Support and Training: Backed by comprehensive technical support, training resources, and expertise to assist users in optimizing assay conditions, troubleshooting issues, and maximizing the performance of the ampPCR panels.

Description

These ampPCR panels incorporate optimized master mixes containing all essential components for the qPCR reaction, including DNA polymerase, dNTPs, buffer, and specific primers and probes tailored to the target of interest. The superior performance of ampPCR panels are attributed to their precise formulation, which ensures minimal variability, high efficiency, and broad dynamic range, enabling reliable detection and quantification of low-abundance targets across a wide range of sample concentrations. Additionally, the integration of advanced fluorescent detection chemistries and optimized thermal cycling protocols further enhances the sensitivity, specificity, and reproducibility of qPCR assays, making them indispensable tools for a myriad of applications in research and biotechnology.

These real-time PCR multiplexed tests ensure accurate and efficient pathogen detection and differentiation of its intended target(s).

Intended use: Available for research use only (RUO). Not for use in diagnostic procedures.

Documents

Wound

⬇️ Download Instructions for Use (IFU)

⬇️ Download Material Safety Data Sheet (MSDS)

Frequently Asked Questions

100 reactions

• Morganella morganii
• Klebsiella pneumoniae
• Staphylococcus aureus
• Enterobacter cloacae
• Enterococcus faecalis
• Proteus mirabilis
• Klebsiella oxytoca
• Pseudomonas aeruginosa
• Escherichia coli
• Serratia marcescens
• Providencia stuartii
• Acinetobacter baumannii
• Bacteroides spp.
• Citrobacter freundii
• Citrobacter braakii
• Klebsiella aerogenes
• Enterococcus faecium
• Proteus vulgaris
• Streptococcus pyogenes

The kit is valid for 12 months after the manufactured date and should be stored frozen at -20±5°C.

We recommend running at least 2 sets of positive and negative controls in case one set fails.
A set refers to running a positive and negative control for every PCR mix included in a detection panel.

Each of the 19 targets are split up into ten reaction mixes. Patient samples and controls are run on each reaction mix so that all targets can be detected.

• 96 well plate: 7 patient samples
• 384 well plate: 36 patient samples

*Running 1 positive control and 1 negative control for each PCR mix. We recommend 2 sets but you only need 1 set to maximize your samples per plate.

The method is estimated to be about ~60 minutes. Some thermal cyclers will vary in the duration of the method.

This kit has a sensitivity as low as 1 copy/uL, but will vary depending on your thermal cycler.

FAM and HEX

It is best to prepare your plate and reagents as needed and not to let it sit at room temperature for extended periods of time. You should avoid storing the PCR reaction if possible; if it is needed, store the PCR reaction at -20°C for up to 30 minutes.

• Positive Control: 1.0mL
• Negative Control: 1.0mL
• PCR mix (10 total): 1.5mL each