Universal Antibiotic Resistance qPCR Kit, 100 Rxn/Kit

Name: Universal Antibiotic Resistance qPCR Kit, 100 Rxn/Kit
Brand: Filtrous
SKU: OSK-TR-0100
Price: 1200.0 USD
Availability: InStock

SKU OSK-TR-0100

by Filtrous

$1,200.00 - $1,200.00

$1,200.00

$1,200.00 - $1,200.00

$1,200.00

Specifications

Specification	Value
Reactions	100 reactions per unit
Targets	MecA MecC VanA/VanB KPC CTX-M
Shelf Life	12 months from the date of manufacture; store frozen at -20°C ± 5°C
Intended use	For use by qualified laboratory personnel for the qualitative detection of antibiotic resistance genes in urine and dry swab samples. Not for diagnostic procedures.

Features and Benefits

Ultra-High Sensitivity: Capable of detecting target concentrations as low as 1 copy/µL, ensuring reliable detection of low-abundance targets.
Exceptional Specificity: Utilizes specific primer and probe sets for each target, minimizing cross-reactivity and ensuring accurate results.
Rapid Turnaround Time: Optimized protocols enable quick amplification and detection, reducing assay times compared to traditional methods.
Multiplexing Capability: Enables simultaneous detection of multiple targets in a single reaction, improving efficiency without compromising sensitivity.
Integrated Quality Controls: Includes internal controls to monitor assay performance and validate results.
High Compatibility: Compatible with major qPCR instruments, facilitating easy integration into laboratory workflows.
Comprehensive Support and Training: Backed by technical support and training resources to optimize assay performance and troubleshoot issues.

Description

These ampPCR panels incorporate optimized master mixes containing all essential components for the qPCR reaction, including DNA polymerase, dNTPs, buffer, and specific primers and probes tailored to the target of interest. The superior performance of ampPCR panels are attributed to their precise formulation, which ensures minimal variability, high efficiency, and broad dynamic range, enabling reliable detection and quantification of low-abundance targets across a wide range of sample concentrations. Additionally, the integration of advanced fluorescent detection chemistries and optimized thermal cycling protocols further enhances the sensitivity, specificity, and reproducibility of qPCR assays, making them indispensable tools for a myriad of applications in research and biotechnology.

These real-time PCR multiplexed tests ensure accurate and efficient pathogen detection and differentiation of its intended target(s).

Intended use: Available for research use only (RUO). Not for use in diagnostic procedures.

Documents

Antibiotic Resistance (ABR)

⬇️ Download Instructions for Use (IFU)

Frequently Asked Questions

100 reactions

• MecA
• MecC
• VanA/VanB
• KPC
• CTX-M

The kit is valid for 12 months after the manufactured date and should be stored frozen at -20±5°C.

We recommend running at least 2 sets of positive and negative controls in case one set fails.
A set refers to running a positive and negative control for every PCR mix included in a detection panel.

Each of the 5 targets are split up into two reaction mixes. Patient samples and controls are run on each reaction mix so that all targets can be detected.

• 96 well plate: 46 patient samples
• 384 well plate: 190 patient samples

*Running 1 positive control and 1 negative control for each PCR mix. We recommend 2 sets but you only need 1 set to maximize your samples per plate.

The method is estimated to be about ~60 minutes. Some thermal cyclers will vary in the duration of the method.

This kit has a sensitivity as low as 1 copy/uL, but will vary depending on your thermal cycler.

FAM, HEX, ROX

It is best to prepare your plate and reagents as needed and not to let it sit at room temperature for extended periods of time. You should avoid storing the PCR reaction if possible; if it is needed, store the PCR reaction at -20°C for up to 30 minutes.

• Positive Control: 200uL
• Negative Control: 200uL
• PCR mix (2 total): 1.5mL each