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20x SSPE

Original price $26 - Original price $65
Original price
$26 - $65
Current price $26
Choose Quantity and Volume

1x Composition:
-150 mM NaCl
-10 mM NaH2PO4
-1 mM EDTA


20x SSPE (sodium chloride-sodium phosphate-EDTA) is a concentrated solution used in molecular biology for nucleic acid hybridization and washing steps. It contains sodium chloride, sodium phosphate, and EDTA at twenty times the normal concentration found in standard SSPE solutions. SSPE provides stringent conditions for nucleic acid binding, helping to minimize nonspecific interactions during hybridization. Typically diluted before use, it ensures optimal stringency levels for specific and sensitive nucleic acid detection. 20x SSPE is a critical reagent in techniques such as Southern blotting and in situ hybridization, enabling precise analysis of nucleic acid sequences.