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10x Tris HEPES SDS Running Buffer

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Original price $65 - Original price $163
Original price
$65
$65 - $163
Current price $65
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Composition:
1x: Tris-HEPES SDS Running Buffer: Tris 0.1M, HEPES 0.1M, SDS 3mM, pH 8

Storage:
4°C recommended

Description

10x Tris HEPES SDS Running Buffer is a concentrated solution used in polyacrylamide gel electrophoresis (PAGE) for protein separation. It contains Tris, HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), and SDS (sodium dodecyl sulfate). Tris and HEPES act as buffering agents, maintaining a stable pH environment during electrophoresis. SDS denatures proteins and confers a negative charge, enabling their migration through the gel based on size. Typically diluted before use to a working concentration, this buffer system is vital for accurate protein analysis in biochemical and molecular biology research.

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