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10x Tris-Glycine Transfer Buffer

Original price $26 - Original price $65
Original price
$26 - $65
Current price $26
Choose Quantity and Volume

-1xTG: Tris 25 mM
-Glycine 192 mM
-pH 8.3, with 20% methanol.


10x Tris-Glycine Transfer Buffer is a concentrated solution utilized in Western blotting for the transfer of proteins from polyacrylamide gels to membranes. It comprises Tris and glycine, which maintain pH stability and facilitate efficient protein migration. This buffer system is crucial for preserving protein integrity during transfer and ensuring accurate detection on the membrane. Typically diluted before use, it provides optimal conditions for the transfer of proteins of varying sizes. Tris-glycine transfer buffer is a fundamental component of Western blot protocols in molecular biology and biochemistry research.