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Try A Free Sample Of Our Stable And Specific GFP-nAb For Your Scientific Research Today!
Accurate Pulldowns Every Time With Our Proprietary GFP-nAb
The GFP-nAb Spin Kit offers a simple, consistent protocol to cleanly pull down GFP fusions. Each GFP-nAb Spin Kit comes with all required buffers and spin columns to allow you to perform multiple immunoprecipitation reactions in a matter of minutes.
Performance and evaluation of GFP using the GFP-nAb Spin Kit. Equal volumes of lysate input (I), flow-through (FT) after binding to GFP-nAb agarose resin, and elution (E) fractions were analyzed by SDS-PAGE followed by Coomassie staining. GFP quantitatively separated from lysate.
Enhanced Performance
Stable up to 70°C, functional in high salt concentrations, or 0.5% SDS
Resilient
Stable up to 70°C, functional in high salt concentrations, or 0.5% SDS
Compact Structure
nAbs are as small as 10kDa enabling efficient pulldown
Biomolecule
Nano-antibody
Conjugate
Agarose; bead size: 50-100μm
Storage Buffer
20% ethanol, 25mM NaPO4, 50mM NaCl
Storage Condition
Shipped at room temperature. Upon receiving, store at 4°C. Stable for one year. Do not freeze.
Specificity/target
Sirius, Azurite, EBFP2, mKalama1, ECFP, mECFP, Cerulean, mCerulean, SCFP3A, CyPet, mTurquoise, mTurquoise2, Sapphire, T-Sapphire, mAmetrine, GFPuv, Superfolder GFP, Emerald, mEmerald, EGFP, mEmerald, Clover, EYFP, mEYFP, Topaz, mTopaz, Venus, mVenus, SYFP2, Citrine, mCitrine, Ypet
What's Included
nAb
20 reactions (500µL)
Lysis Buffer
20mM Tris-HCl pH 7.5, 150mM NaCl, 1mM EDTA, 0.5% NP-40
Wash Buffer
10mM Tris-HCl pH 7.5, 500mM NaCl
Elution Buffer
200mM glycine pH 2.5
Neutralization Buffer
1M Tris Base
Binding Buffer
10mM Tris-HCl pH 7.5, 150mM NaCl
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How To Use nAbs At a Glance
Instructional nAb Video For Your Research
nAb Fluorescent Protein Specificity
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