Setup Loops with Tecan EVOware Software: A Step-by-Step Guide (2023)
If you're a lab rat who's been wrestling with pipetting tasks, then this guide is for you! We're going to dive into the world of Tecan EVOware software and unravel the mystery of using loops for pipetting. So, buckle up, grab your lab consumables, and let's get started!
The GADD Principle
In the realm of pipetting, there's a sacred acronym that you need to etch into your memory - GADD. It stands for Get tips, Aspirate, Dispense, and Drop tips. These are the four commandments of pipetting, and they're going to be your best friends when using the Tecan EVOware software.
Pipetting with a 96-Well Plate
First up, let's tackle the 96-well plate. For this, you'll need your trusty 1000 microliter filter tips. These are the workhorses of your pipetting tasks, and they're ready to get down to business.
In the software, you'll need to set the loop to vary the column by one for each cycle. This is like a choreographed dance for your pipette, moving it gracefully from one well to the next. As for the volume, we're going with 250 microliters for both aspiration and dispensing. Remember, precision is key in the lab!
Pipetting with a 384-Well Plate
Now, let's turn our attention to the 384-well plate. This is where things get a bit more intricate. You'll need to swap out your 1000 microliter tips for something a bit more delicate - the 50 microliter tips.
The tip spacing needs to be adjusted here. Think of it as social distancing for your pipette tips. You'll want to fill the plate across rather than down, as this is the fast lane in the world of pipetting.
Next, set the software to vary the row by one after completing a loop. This is like a game of snake, filling the plate row by row. It's a meticulous process, but with the Tecan EVOware software, it's a breeze.
To wrap things up, let's revisit our loop options. Make sure you've set your loop to vary the column and row correctly. It's like setting the coordinates for your pipetting journey.
And there you have it! You're now a whizz at using loops in the Tecan EVOware software for your pipetting tasks. So, go forth, lab warriors, and pipette with confidence!
Remember, whether you're dealing with 96-well plates or 384-well plates, whether you're aspirating or dispensing, the key is in the details. And with the right lab consumables and the Tecan EVOware software, you're well on your way to mastering the art of pipetting.
Stay tuned for more lab hacks and remember, science is not just about the destination, it's about the journey. Happy pipetting!