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ampPCR Urinary Tract Infection qPCR Detection Panel, 100 Reactions/unit

SKU OSK-UT-0100

Specifications

Specification Value
Product Type Urinary Tract Infection qPCR Detection Panel
Reactions 100 reactions
Application qPCR
Purpose Detection of urinary tract infections
Storage Store at -20°C
Sensitivity
1 copy/uL
Time to Results
45-55 minutes
Compatibility

Including but not limited to 96 and 384 versions of
- QuantStudio 5, 7 and 12
- Roche LightCycler II
- BioRad CFX and Opus

    Description

    Culture-based methods for the detection of UTI pathogens lack sensitivity, are subjective, and prolonged Turnaround Times (48-72 Hours). Clinicians frequently treat patients empirically resulting in poor antibiotic stewardship and increased rate of antibiotic resistance. Our new real-time PCR solution for UTI investigations enables rapid detection without the need of culture—while enabling increased sensitivity and specificity of detection of urinary tract pathogens and keeping the cost per sample low. Our real-time PCR assays use DNA isolated from specimens such as urine or blood. The product is intended for use by qualified laboratory personnel well-trained in nucleic acid amplification techniques and in vitro diagnostic procedures. Positive results do not rule out bacterial infection or co-infection with other pathogens. Negative results do not preclude microbial infection and should not be used as the sole basis for patient management decisions. Test results must be combined with clinical observations, patient history, and epidemiological information.

    Targets:

    • Enterobacter cloacae
    • Enterococcus faecalis
    • Escherichia coli
    • Klebsiella oxytoca
    • Klebsiella pneumoniae
    • Morganella morganii
    • Mycoplasma hominis
    • Proteus mirabilis
    • Providencia stuartii
    • Pseudomonas aeruginosa
    • Serratia marcescens
    • Staphylococcus aureus
    • Staphylococcus saprophyticus
    • Streptococcus agalactiae
    • Ureaplasma parvum serovar
    • Ureaplasma urealyticum
    • C. albicans
    • C. glabrata
    • C. parapsilosis
    • C. tropicalis

    Comprehensive Detection: Covers a wide range of urinary tract infection bacteria targets.

    Efficient: Contains material for 100 reactions, ideal for medium to large-scale testing.

    High Sensitivity and Specificity: Utilizes PCR technology, known for its high sensitivity and specificity in pathogen detection.

    Versatile: Suitable for clinical laboratories, research institutions, and public health settings.

    For Research Use Only. Not for use in diagnostic procedures.

    The UTI qPCR Detection Panel provides a highly efficient and reliable method for detecting a broad range of 20 urinary tract infection bacteria targets, including Enterobacter cloacae, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa, among others.

    This kit contains material for 100 reactions, making it a perfect fit for medium to large-scale testing applications in clinical laboratories, research institutions, and public health settings. The PCR technology employed ensures high sensitivity and specificity, giving you confidence in the accuracy of your results.

    For optimal performance, the UTI qPCR Detection Panel should be stored at -20°C when not in use. The fight against urinary tract infections starts with accurate detection, and our UTI RT-PCR Detection Panel is the trusted choice for comprehensive detection.

    Frequently Asked Questions

    How many reactions are in the kit?

    100 reactions


    What is the shelf life of the kit?

    The kit is valid for 12 months after the manufactured date or until the expiration date and should be stored in a -20°C storage


    How many controls should I run on a plate for my clinical samples?

    We recommend running at least 2 sets of positive and negative controls in case one set fails (a set refers to running a positive and negative for ALL oligo mixes).


    How many patient samples can I do in a single run?

    96 Well Plate: 9 Patient Samples (assuming you are running 2 sets of positive and negative controls)
    384 Well Plate: 50 Patient Samples (assuming you are running 2 sets of positive and negative controls and filling all the plate wells)


    What is the estimated run time of the method with this kit?

    The method is estimated to be about ~60 minutes. Some thermal cyclers will vary in the duration of the method.


    What is the LOD for this kit?

    This kit has a sensitivity as low as 1 cp/uL, but will vary depending on your thermal cycler.


    What dyes should my thermal cycler be calibrated for?

    FAM, JOE, ROX, Cy5, SYBR


    What is the stability of the prepared PCR plate? Am I able to store it in a freezer after making the reaction?

    It is best to prepare your plate and reagents as needed and not to let it sit at room temperature for extended periods of time. You should avoid storing the PCR reaction if possible; if it is needed, store the PCR reaction at -20°C for up to 30 minutes.


    What are quantities of control and PCR mix?

    Positive control: 100uL
    Negative Control: 100uL
    UTI(1-7) PCR Mix: 1.5mL each tube

    Turnkey validation

    Comprehensive solution for your lab's validation project. Reduce launch time by 80% or more.

    Schedule a Consultation

    Automation Compatible

    Ready to use with QuantStudio 5, 7, 12, Roche LightCycler II, and BioRad CFX and Opus

    High Sensitivity

    Detects 1 copy of viral RNA per microliter of transport media

    Get results. Fast.

    Get results. Fast.

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