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ampPCR Pneumonia qPCR Detection Panel, 100 Reactions/unit



Specification Value
Product Type Pneumonia qPCR Detection Panel
Reactions 100 reactions
Application qPCR
Purpose Detection of pneumonia
Storage Store at -20°C
1 copy/uL
Time to Results
45-55 minutes

Including but not limited to 96 and 384 versions of
- QuantStudio 5, 7 and 12
- Roche LightCycler II
- BioRad CFX and Opus


    Respiratory pathogens cause acute local and systemic disease, such as pneumonia. Due to the similarity of diseases caused by many bacteria or fungi, diagnosis based on clinical symptoms alone is difficult. Identification of potential causative agents provides data to aid the physician in determining appropriate patient treatment and public health response for disease containment. This panel is designed for specific and qualitative detection of pneumonia-causing bacteria in specimens such as oropharyngeal swabs, nasopharyngeal swabs or sputum. The product is intended for use by qualified laboratory personnel well trained in nucleic acid amplification techniques and in vitro diagnostic procedures. Positive results do not rule out bacterial infection or co-infection with other pathogens. Negative results do not preclude microbial infection and should not be used as the sole basis for patient management decisions. Test results must be combined with clinical observations, patient history, and epidemiological information.


    • Haemophilus influenzae
    • Klebsiella aerogenes
    • Klebsiella pneumonia group
    • Moraxella catarrhalis
    • Pseudomonas aeruginosa
    • Serratia marcescens
    • Staphylococcus aureus
    • Streptococcus agalactiae
    • Streptococcus pneumoniae
    • Streptococcus pyogenes
    • Chlamydia pneumoniae
    • Legionella pneumophila
    • Mycoplasma pneumoniae

    The Pneumonia qPCR Detection Panel is specifically designed to detect and identify multiple pneumonia-causing pathogens. It targets specific genes associated with these pathogens, enabling a comprehensive analysis for accurate pneumonia diagnosis.

    Utilizing the sensitive and specific Reverse Transcription Polymerase Chain Reaction (qPCR) method, this detection panel ensures reliable detection of pneumonia pathogens. It offers high sensitivity, even for low levels of pathogen DNA in respiratory specimens.

    The detection panel is compatible with standard qPCR instruments, making it accessible to a wide range of laboratories. It seamlessly integrates into existing laboratory workflows, eliminating the need for specialized equipment.

    The Pneumonia qPCR Detection Panel includes positive and negative controls for assay validation and quality control. These controls ensure the reliability and accuracy of the test results, providing confidence in the detection process.

    The Pneumonia qPCR Detection Panel is a powerful solution for the accurate and efficient detection of pneumonia-causing pathogens. Specifically designed for pneumonia diagnosis, this panel utilizes the sensitive and specific Reverse Transcription Polymerase Chain Reaction (qPCR) method to identify multiple pathogens associated with pneumonia.

    With a capacity of 100 reactions per run, this detection panel optimizes laboratory efficiency by enabling rapid and simultaneous analysis of respiratory specimens. It is compatible with standard qPCR instruments, allowing seamless integration into existing laboratory workflows without the need for additional equipment.

    Manufactured under an ISO 13485 quality management system, the Pneumonia qPCR Detection Panel meets the highest standards of quality and regulatory compliance. It complies with applicable regulatory requirements, providing assurance in its performance and reliability.

    Choose the Pneumonia qPCR Detection Panel for accurate and efficient detection of pneumonia-causing pathogens. With its advanced technology and user-friendly design, it empowers healthcare professionals in their efforts to diagnose and manage pneumonia effectively, ultimately improving patient outcomes.

    Frequently Asked Questions

    How many reactions are in the kit?

    100 reactions

    What is the shelf life of the kit?

    The kit is valid for 12 months after the manufactured date or until the expiration date and should be stored in a -20°C storage.

    How many controls should I run on a plate for my clinical samples?

    We recommend running at least 2 sets of positive and negative controls in case one set fails (a set refers to running a positive and negative for ALL oligo mixes).

    Why are there four reaction mixes?

    Each of the 11 targets are split up into four reaction mixes. Patient samples and controls are run on each reaction mix so that all targets can be detected.

    How many patient samples can I do in a single run?

    96 Well Plate: 20 Patient Samples (assuming you are running 2 sets of positive and negative controls)
    384 Well Plate: 92 Patient Samples (assuming you are running 2 sets of positive and negative controls and filling all the plate wells)

    What is the estimated run time of the method with this kit?

    The method is estimated to be ~60 minutes. Some thermal cyclers will vary in the duration of the method.

    What is the LOD for this kit?

    This kit has a sensitivity as low as 0.2 cps/uL, but will vary depending on your thermal cycler.

    What dyes should my thermal cycler be calibrated for?

    FAM, JOE, ROX, Cy5

    What is the stability of the prepared PCR plate? Am I able to store it in a freezer after making the reaction?

    Due to the nature of some RNA based analytes, it is best to prepare your plate and reagents as needed and not to let it sit at room temperature for extended periods of time. You should avoid storing the PCR reaction if possible, if it is needed it is store the PCR reaction at -20°C for up to 30 minutes.

    What are the quantities of control and PCR mix?

    Positive Control: 200uL
    Negative Control: 200uL
    Pn(1-4) PCR Mix: 1.6mL each tube

    Turnkey validation

    Comprehensive solution for your lab's validation project. Reduce launch time by 80% or more.

    Schedule a Consultation

    Automation Compatible

    Ready to use with QuantStudio 5, 7, 12, Roche LightCycler II, and BioRad CFX and Opus

    High Sensitivity

    Detects 1 copy of viral RNA per microliter of transport media

    Get results. Fast.

    Get results. Fast.

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