{"product_id":"pmaxx™-bottled-treatment-kit-biotium-200-preps-unit","title":"PMAxx™ Bottled Treatment Kit, Biotium, 200 Preps\/unit","description":"\u003c!-- FILTROUS:SPECS --\u003e\n\u003ctable class=\"fpd__specs-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eReagent\u003c\/td\u003e\n\u003ctd\u003eBiotium PMAxx™ Dye, 20 mM in water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVolume\u003c\/td\u003e\n\u003ctd\u003e100 µL, sealed vial\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePreps\u003c\/td\u003e\n\u003ctd\u003e200 (0.5 µL per 400 µL sample at 25 µM)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplication\u003c\/td\u003e\n\u003ctd\u003eViability PCR of bacterial samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTreatment\u003c\/td\u003e\n\u003ctd\u003e10 minutes in the dark at room temperature, then a 15-minute blue LED light exposure\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsorption \/ emission\u003c\/td\u003e\n\u003ctd\u003e464 nm before photolysis; 510 \/ 610 nm after photolysis and reaction with DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLive-dead separation\u003c\/td\u003e\n\u003ctd\u003eA further 3–7 Ct compared with PMA (Biotium data)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e–20 °C, protected from light; stable for at least 6 months from receipt\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTrademark\u003c\/td\u003e\n\u003ctd\u003ePMAxx is a trademark of Biotium, Inc.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUse\u003c\/td\u003e\n\u003ctd\u003eResearch Use Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSKU\u003c\/td\u003e\n\u003ctd\u003eEXK-PX-0200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c!-- \/FILTROUS:SPECS --\u003e\n\u003c!-- FILTROUS:DESC --\u003e\n\u003cp style=\"margin-top:0\"\u003eThe Filtrous EXK-PX-0200 PMAxx™ Bottled Treatment Kit is a sealed 100 µL vial of Biotium PMAxx™ Dye at 20 mM in water, enough for 200 viability PCR treatments of 400 µL bacterial samples at 25 µM. PMAxx™ is a photoreactive, membrane-impermeant DNA-binding dye: it enters only cells with compromised membranes, and after a blue-light exposure it covalently modifies their DNA so it drops out of the PCR.\u003c\/p\u003e\n\u003cp\u003eDNA in viable cells stays intact, so the qPCR signal comes from live cells, and Biotium's data show a further 3–7 Ct of live-dead separation compared with PMA. Treatment is 10 minutes in the dark at room temperature, a 15-minute LED light exposure and a 5,000 × g spin, followed by extraction with any DNA kit and equal eluate volumes into qPCR rather than DNA normalized by mass. It suits viability qPCR of bacterial cultures, enrichments and environmental samples with primers for an amplicon of at least 100 bp, for Research Use Only.\u003c\/p\u003e\n\u003c!-- \/FILTROUS:DESC --\u003e","brand":"Filtrous","offers":[{"title":"Default Title","offer_id":67691445747986,"sku":"EXK-PX-0200","price":1100.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/filtrous.com\/products\/pmaxx%e2%84%a2-bottled-treatment-kit-biotium-200-preps-unit","provider":"Filtrous","version":"1.0","type":"link"}