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Lumira One Step Universal COVID qPCR Detection Panel, 25uL

SKU OSK-LX-0384

Specifications

Specification Value
Product Type One-Step Universal qPCR Kit
Reactions 384 reactions
Reaction Volume 25µL
Application qPCR
Ideal for Isolating total viral RNA, COVID-19 detection
Validation EUA-FDA validated
Turn-around Time Under 25 minutes
Sensitivity
1 copy/uL
Time to Results
45-55 minutes
Compatibility

Including but not limited to 96 and 384 versions of
- QuantStudio 5, 7 and 12
- Roche LightCycler II
- BioRad CFX and Opus


    Description

    Compact Yet Efficient: 384 reactions with 25µL reaction volume per kit, offering a balance of efficiency and compactness.

    Universal Application: Suitable for isolating total viral RNA from a variety of specimens, including plasma, serum, and various types of swabs and aspirates.

    EUA-FDA Validated: Authorized for use with swab specimens collected from any individual, not limited to those showing symptoms or suspected of COVID-19.

    Efficient and Fast: Direct amplification in under 25 minutes, significantly increasing lab's productivity.

    Ready-to-Use: Comes in pre-filled plates, ready for sample addition.

    Reliable: Stringently developed and tested for reproducibility and consistency.

    Automation Compatible: Compatible with major automation platforms, enhancing throughput and ease-of-use.

    The One-Step Universal qPCR Kit with a 25µL reaction volume provides a compact yet efficient solution for your lab. Each kit supports 384 reactions, making it an ideal choice for moderate-throughput settings or studies.

    This EUA-FDA validated kit is approved for use with swab specimens collected from any individual, not just those showing symptoms or suspected of COVID-19. It isolates total viral RNA from various samples and offers direct amplification in less than 25 minutes. The pre-filled plates are ready for your samples, simplifying your workflow and saving you valuable time.

    Developed and tested under stringent conditions, this kit ensures reproducibility and consistency. Furthermore, it is compatible with major automation platforms, making it an asset to any modern laboratory.

    Embrace the balance of compactness and efficiency with our One-Step Universal qPCR Kit. It's your reliable partner for achieving accurate detection, reduced complexity, and fast turn-around times in your lab operations. Start your journey to higher productivity with us today.

    Compact Yet Efficient: 384 reactions with 25µL reaction volume per kit, offering a balance of efficiency and compactness.

    Universal Application: Suitable for isolating total viral RNA from a variety of specimens, including plasma, serum, and various types of swabs and aspirates.

    Frequently Asked Questions

    How many reactions are in the kit?

    384 reactions


    What is the shelf life of the kit?

    The kit is valid until the expiration date and should be stored in a -20°C storage.


    How many controls should I run on a plate for my clinical samples?

    We recommend running at least 2 sets of positive and negative controls in case one set fails (a set refers to running a positive and negative for ALL oligo mixes).


    How many patient samples can I do in a single run?

    96 Well Plate: 92 Patient Samples (assuming you are running 2 sets of positive and negative controls)
    384 Well Plate: 380 Patient Samples (assuming you are running 2 sets of positive and negative controls and filling all the plate wells)


    What is the estimated run time of the method with this kit?

    The method is estimated to be about ~30 minutes. Some thermal cyclers will vary in the duration of the method.


    What is the LOD for this kit?

    This kit has a sensitivity as low as 2.5 cp/uL, but will vary depending on your thermal cycler.


    What dyes/channels should my thermal cycler be calibrated for?

    FAM and ROX


    What is the stability of the prepared PCR plate? Am I able to store it in a freezer after making the reaction?

    These PCR reactions for the Lumira kit are heat-sensitive. Use a cold block when creating and adding master mix. Premade reagents (such as a mixture between IC/P, Salt Mix, and Master Mix) should be made as needed and may should be stored in -20°C for up to 48 hours.

    What are quantities of control and PCR mix?

    Positive control: 500uL
    Negative Control: 1.5mL
    Master Mix: 2x 1mL
    Internal Control & Primer Mix (IC/P Mix): 120uL
    Salt Mix: 1mL
    Extraction Buffer: 500uL

    Turnkey validation

    Comprehensive solution for your lab's validation project. Reduce launch time by 80% or more.

    Schedule a Consultation

    Automation Compatible

    Ready to use with QuantStudio 5, 7, 12, Roche LightCycler II, and BioRad CFX and Opus

    High Sensitivity

    Detects 1 copy of viral RNA per microliter of transport media

    Get results. Fast.

    Get results. Fast.

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