How many reactions are in the kit?
What is the shelf life of the kit?
The kit is valid for 12 months after the manufactured date or until the expiration date and should be stored in a -20°C storage.
How many controls should I run on a plate for my clinical samples?
We recommend running at least 2 sets of positive and negative controls in case one set fails (a set refers to running a positive and negative for ALL oligo mixes).
How many patient samples can I do in a single run?
96 Well Plate: 92 Patient Samples (assuming you are running 2 sets of positive and negative controls)
384 Well Plate: 380 Patient Samples (assuming you are running 2 sets of positive and negative controls and filling all the plate wells)
What is the estimated run time of the method with this kit?
The method is estimated to be about ~70 minutes. Some thermal cyclers will vary in the duration of the method.
What is the LOD for this kit?
This kit has a sensitivity as low as 1 cp/uL, but will vary depending on your thermal cycler.
What dyes should my thermal cycler be calibrated for?
FAM, JOE, ROX, Cy5
What is the stability of the prepared PCR plate? Am I able to store it in a freezer after making the reaction?
It is best to prepare your plate and reagents as needed and not to let it sit at room temperature for extended periods of time. You should avoid storing the PCR reaction if possible; if it is needed, store the PCR reaction at -20°C for up to 30 minutes.
What are quantities of control and PCR mix?
Positive control: 100uL
Negative Control: 100uL
RT PCR Enzyme Mix: 110uL
PCR Mix: 1.5mL